Inhibition of miR-223 Expression Using a Sponge Strategy Decreases Restenosis in Rat Injured Carotids - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Current Vascular Pharmacology Année : 2020

Inhibition of miR-223 Expression Using a Sponge Strategy Decreases Restenosis in Rat Injured Carotids

(1) , (2) , (3) , , , (4) , (5) , (6, 7, 8) , (9) , (10, 3) , (11, 3)
1
2
3
4
5
6
7
8
9
10
11
Noura Bahrar
  • Fonction : Auteur
Tanja Celic
  • Fonction : Auteur

Résumé

Objective: Restenosis is a frequent complication of angioplasty. It consists of a neointimal hyperplasia resulting from progression and migration of vascular smooth muscle cells (VSMC) into the vessel lumen. microRNA miR-223 has recently been shown to be involved in cardiovascular diseases including atherosclerosis, vascular calcification and arterial thrombosis. In this study, our aim was to assess the impact of miR-223 modulation on restenosis in a rat model of carotid artery after balloon injury. Methods: The over and down-expression of miR-223 was induced by adenoviral vectors, containing either a pre-miR-223 sequence allowing artificial miR-223 expression or a sponge sequence, trapping the native microRNA, respectively. Restenosis was quantified on stained rat carotid sections. Results: In vitro, three mRNA (Myocyte Enhancer Factor 2C (MEF2C), Ras homolog gene family, member B (RhoB) and Nuclear factor 1 A-type (NFIA)) reported as miR-223 direct targets and known to be implicated in VSMC differentiation and contractility were studied by RT-qPCR. Our findings showed that down-expression of miR-223 significantly reduced neointimal hyperplasia by 44% in carotids, and was associated with a 2-3-fold overexpression of MEF2C, RhoB and NFIA in a murine monocyte macrophage cell line, RAW 264.7 cells. Conclusion: Down-regulating miR-223 could be a potential therapeutic approach to prevent restenosis after angioplasty.
Fichier non déposé

Dates et versions

hal-03583942 , version 1 (22-02-2022)

Identifiants

Citer

Eleonore M'Baya-Moutoula, Alexandre Marchand, Isabelle Six, Noura Bahrar, Tanja Celic, et al.. Inhibition of miR-223 Expression Using a Sponge Strategy Decreases Restenosis in Rat Injured Carotids. Current Vascular Pharmacology, 2020, 18 (5), pp.507-516. ⟨10.2174/1570161117666190705141152⟩. ⟨hal-03583942⟩
16 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook Twitter LinkedIn More