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An Alginate-Based Hydrogel with a High Angiogenic Capacity and a High Osteogenic Potential

Abstract : In bone tissue engineering, autologous cells are combined with osteoconductive scaffolds and implanted into bone defects. The major challenge is the lack of post-implantation vascular growth into biomaterial. The objective of the present study was to develop a new alginate-based hydrogel that enhances the regeneration of bone defects after surgery. The viability of human bone marrow-derived mesenchymal stem cells (BM-MSCs) or human endothelial cells (ECs) cultured alone or together on the hydrogel was analyzed for 24 and 96 h. After seeding, the cells self-assembled and aggregated to form clusters. For functional validation, empty or cellularized hydrogel matrices were implanted ectopically at subcutaneous sites in nude mice. After 2 months, the matrices were explanted. Transplanted human cells were present, and we observed vessels expressing human von Willebrand factor (resulting from the incorporation of transplanted ECs into neovessels and/or the differentiation of BM-MSCs into ECs). The addition of BM-MSCs improved host vascularization and neovessel formation from human cells, relative to ECs alone. Although we did not observe bone formation, the transplanted BM-MSCs were able to differentiate into osteoblasts. This new biomaterial provided an appropriate three-dimensional environment for transplanted cells and has a high angiogenic capacity and an osteogenic potential.
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https://hal-u-picardie.archives-ouvertes.fr/hal-03598573
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Soumis le : samedi 5 mars 2022 - 14:49:43
Dernière modification le : mardi 15 mars 2022 - 03:22:42

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Anais Barre, Marie Naudot, Fanny Colin, Henri Sevestre, Louison Collet, et al.. An Alginate-Based Hydrogel with a High Angiogenic Capacity and a High Osteogenic Potential. BIORESEARCH OPEN ACCESS, 2020, 9 (1), pp.174-182. ⟨10.1089/biores.2020.0010⟩. ⟨hal-03598573⟩

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