Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Timing of TolA and TolQ recruitment at the septum depends on the functionality of the Tol-Pal system

Abstract : Efficient cell division of Gram-negative bacteria requires the presence of the Tol-Pal system to coordinate outer membrane (OM) invagination with inner membrane invagination (IM) and peptidoglycan (PG) remodeling. The Tol-Pal system is a transenvelope complex that connects the three layers of the cell envelope through an energy-dependent process. It is composed of the three IM proteins, TolA, TolQ and TolR, the periplasmic protein TolB and the OM lipoprotein Pal. The proteins of the Tol-Pal system are dynamically recruited to the cell septum during cell division. TolA, the central hub of the Tol-Pal system, has three domains: a transmembrane helix (TolA1), a long second helical periplasmic domain (TolA2) and a C-terminal globular domain (TolA3). The TolQR complex uses the PMF to energize TolA, allowing its cyclic interaction via TolA3 with the OM TolB-Pal complex. Here, we confirm that TolA2 is sufficient to address TolA to the site of constriction, whereas TolA1 is recruited by TolQ. Analysis of the protein localization as function of the bacterial cell age revealed that TolA and TolQ localize earlier at midcell in the absence of the other Tol-Pal proteins. These data suggest that TolA and TolQ are delayed from their septal recruitment by the multiple interactions of TolA with TolB-Pal in the cell envelope providing a new example of temporal regulation of proteins recruitment at the septum.
Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal.archives-ouvertes.fr/hal-03600999
Contributeur : Denis Duché Connectez-vous pour contacter le contributeur
Soumis le : mardi 8 mars 2022 - 08:42:24
Dernière modification le : mercredi 9 mars 2022 - 03:34:31
Archivage à long terme le : : jeudi 9 juin 2022 - 18:26:19

Fichier

 Accès restreint
Fichier visible le : 2022-10-15

Connectez-vous pour demander l'accès au fichier

Licence


Distributed under a Creative Commons Paternité - Pas d'utilisation commerciale - Pas de modification 4.0 International License

Identifiants

Collections

Citation

Pauline Baccelli, Raphaël Rachedi, Bastien Serrano, Mélissa Petiti, Christophe Bernard, et al.. Timing of TolA and TolQ recruitment at the septum depends on the functionality of the Tol-Pal system. Journal of Molecular Biology, Elsevier, In press, 434 (7), pp.167519. ⟨10.1016/j.jmb.2022.167519⟩. ⟨hal-03600999⟩

Partager

Métriques

Consultations de la notice

43