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Article Dans Une Revue European Journal of Mass Spectrometry Année : 2019

High resolution MALDI-TOF-MS and MS/MS: Application for the structural characterization of sulfated oligosaccharides

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Résumé

Sulfated oligosaccharides are involved in important biological events that are often modulated by specific sequences and sulfation patterns, but their structural analysis remains challenging. Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) analysis of three different sulfated oligosaccharides (Fondaparinux, the octasulfated pentasaccharide, a disulfated heparin-derived tetrasaccharide 1, and an octasulfated maltoheptaose) 2 was performed using the 2-(4-hydroxyphenylazo)benzoic acid-tetramethylguanidinium (HABA-TMG(2)) matrix. High resolution mass spectrometry of the main ions observed was successful, and this was complemented by tandem mass spectrometry (MS/MS) analysis for structural assessment. Despite sulfate losses, fully sulfated molecular ions were observed and these allowed the determination of oligosaccharide structures: UA-GlcNAc-UA(2S)-AnhMan(6S) for compound 1 and (Glc6S)(6)-Glc (1S,6S) for compound 2.

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Dates et versions

hal-03613822 , version 1 (18-03-2022)

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David Lesur, Gilbert Duhirwe, Jose Kovensky. High resolution MALDI-TOF-MS and MS/MS: Application for the structural characterization of sulfated oligosaccharides. European Journal of Mass Spectrometry, 2019, 25 (5), pp.428-436. ⟨10.1177/1469066719851438⟩. ⟨hal-03613822⟩
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