Arrêt de service programmé du vendredi 10 juin 16h jusqu’au lundi 13 juin 9h. Pour en savoir plus
Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

High resolution MALDI-TOF-MS and MS/MS: Application for the structural characterization of sulfated oligosaccharides

Abstract : Sulfated oligosaccharides are involved in important biological events that are often modulated by specific sequences and sulfation patterns, but their structural analysis remains challenging. Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) analysis of three different sulfated oligosaccharides (Fondaparinux, the octasulfated pentasaccharide, a disulfated heparin-derived tetrasaccharide 1, and an octasulfated maltoheptaose) 2 was performed using the 2-(4-hydroxyphenylazo)benzoic acid-tetramethylguanidinium (HABA-TMG(2)) matrix. High resolution mass spectrometry of the main ions observed was successful, and this was complemented by tandem mass spectrometry (MS/MS) analysis for structural assessment. Despite sulfate losses, fully sulfated molecular ions were observed and these allowed the determination of oligosaccharide structures: UA-GlcNAc-UA(2S)-AnhMan(6S) for compound 1 and (Glc6S)(6)-Glc (1S,6S) for compound 2.
Type de document :
Article dans une revue
Domaine :
Liste complète des métadonnées

https://hal-u-picardie.archives-ouvertes.fr/hal-03613822
Contributeur : Louise Dessaivre Connectez-vous pour contacter le contributeur
Soumis le : vendredi 18 mars 2022 - 17:47:18
Dernière modification le : samedi 19 mars 2022 - 03:39:49

Identifiants

Collections

Citation

David Lesur, Gilbert Duhirwe, Jose Kovensky. High resolution MALDI-TOF-MS and MS/MS: Application for the structural characterization of sulfated oligosaccharides. EUROPEAN JOURNAL OF MASS SPECTROMETRY, 2019, 25 (5), pp.428-436. ⟨10.1177/1469066719851438⟩. ⟨hal-03613822⟩

Partager

Métriques

Consultations de la notice

5