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Caffeine citrate effects on gastrointestinal permeability, bacterial translocation and biochemical parameters in newborn rats after long-term oral administration

Abstract : BACKGROUND: Caffeine is a potent central and respiratory acting agent used in neonatology to treat apnea in premature newborns. OBJECTIVE: This study investigates the effects of caffeine orally administered to newborn rats on gastrointestinal permeability, bacterial translocation and different biochemical parameters. METHODS: Newborn rats were divided into different groups (N= 06). The treated newborn rats were orally administered with standard caffeine doses (12 mg/kg per day), and the control groups received a placebo. The animals were weighed daily until sacrifice. Blood samples, mesenteric lymph nodes (MLN) and organs were aseptically collected. Furthermore, different biochemical (D-Lactate) and oxidative stress biomarkers (MDA, CAT, SOD and GSH) were examined. Microbiological analyses were performed to assess microbiota alterations and bacterial translocation. RESULTS: Preliminary results showed that caffeine administration decreased the level of bacterial translocation over time. The treatment reduced plasma D-lactate levels (p < 0.05). Additionally, caffeine induced a disturbance in the concentrations of biochemical parameters and oxidative stress biomarkers. Indeed, liver enzymes (AST and ALT) were significantly (p < 0.05) risen after caffeine treatment. Glutathione (GSH) levels were significantly higher in caffeine treated groups (75.12 +/- 0.32; 51.98 +/- 1.12 U/mg; p < 0.05) comparing to control ones (40.82 +/- 0.25; 42.91 +/- 0.27 U/mg; p < 0.05) in the ileum and the colon, respectively. CONCLUSIONS: Thus, besides improving gastrointestinal permeability, our data show that caffeine has beneficial effects on the intestinal antioxidant system.
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https://hal-u-picardie.archives-ouvertes.fr/hal-03791159
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Soumis le : jeudi 29 septembre 2022 - 09:14:41
Dernière modification le : samedi 22 octobre 2022 - 03:06:22

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Foufa Bouabsa, Aicha Tir Touil, Mazhar Salim Al Zoubi, Nadia Chellia, Andre Leke, et al.. Caffeine citrate effects on gastrointestinal permeability, bacterial translocation and biochemical parameters in newborn rats after long-term oral administration. Mediterranean Journal of Nutrition and Metabolism, 2022, 15 (3), pp.307-321. ⟨10.3233/MNM-211544⟩. ⟨hal-03791159⟩

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