GluNs Detection and Functions in Microglial Cells

Stéphane Peineau (1, 2) , Vincent Degos (2, 3) , Catherine Verney , Pierre Gressens (2)

1 GRAP - Groupe de Recherche sur l'alcool et les pharmacodépendances - UMR INSERM_S 1247
2 PROTECT - Neuroprotection du Cerveau en Développement / Promoting Research Oriented Towards Early Cns Therapies
3 CHU Pitié-Salpêtrière [AP-HP]

Proving endogenous GluN presence and functions in microglia require complementary steps to demonstrate (1) that GluN genes are transcripted and translated, (2) their cellular localization, (3) that the GluN are functional, and (4) the role of the functional GluN. The complete demonstration is performed by using mRNA detection technics, western blots, immunofluorescence, electrophysiology, calcium imaging, morphology studies, multiplex immunoassay together with conditional microglial Knock-Out mice and brain lesion models.

Mots clés: Microglia; Brain; NMDA receptor; GluN; Western blots; Immunofluorescence; Electrophysiology; Calcium imaging; Morphology studies; Multiplex immunoassay

Domaines: Neurosciences [q-bio.NC]

Stéphane Peineau, Vincent Degos, Catherine Verney, Pierre Gressens. GluNs Detection and Functions in Microglial Cells. NMDA Receptors, 1677, Springer New York, pp.253-263, 2017, Methods in Molecular Biology, ⟨10.1007/978-1-4939-7321-7_14⟩. ⟨hal-04053529⟩