miR-92a: A Novel Potential Biomarker of Rapid Aortic Valve Calcification

Joseph Nader (1) , Valérie Metzinger-Le Meuth (2, 3) , Pierre Maitrias (1) , Jean-Régis Humbert , Benjamin Brigant (2) , Christophe Tribouilloy (1, 2) , Laurent Metzinger (2) , Thierry Caus (1, 2)

1 CHU Amiens-Picardie
2 MP3CV - Mécanismes physiopathologiques et conséquences des calcifications vasculaires - UR UPJV 7517
3 UP13 - Université Paris 13

Background and aim of the study: The study aim wasto compare the tissular expression of microRNAs (miRs) in bicuspid and tricuspid valves, and to evaluate their use as potential novel biomarkers of aortic valve calcification in bicuspid valves. Methods: A prospective single-center observational study was conducted on stenotic bicuspid andtricuspid human aortic valves. According to their potential role in valve vascular and valvular calcification, a decision was taken to include miR-92a, miR-141, and miR-223 in this analysis. A real-time quantitative polymerase chain reaction was used to measure the expression of each miR, using U6and Cel-miR-39 as endogenous and exogenous genecontrols, respectively. Results: Among a total of 47 human calcified aortic valves collected, 30 (63.8%) were tricuspid valves. Theme an preoperative transvalvular gradient was 50.8mmHg (range: 37-89 mmHg), with no significant difference between bicuspid and tricuspid valves(50 mmHg versus 51.2 mmHg; p = 0.729). Theme an aortic valve area was 0.79 cm2 (range: 0.33-1.3 cm2), again with no significant difference between the two groups (p = 0.34). The level of miR-92a expression was twofold higher in bicuspid valves compared to tricuspid valves (0.38 versus 0.17; p = 0.016), butno significant difference in miR-141 and miR-223 expression was observed between the two groups (p =0.68 and p = 0.35, respectively). A positive correlation was observed between miR-92a expression and mean preoperative transvalvular gradient (r = 0.3257, p =0.04). Conclusion: miR-92a is overexpressed in calcified bicuspid aortic valves, and may serve as a potential biomarker of rapid aortic valve calcification. Further studies based on these results may be designed to correlate the relative expression of miR-92a in the serum with its tissular expression in AS.

Domaines: Médecine humaine et pathologie

Joseph Nader, Valérie Metzinger-Le Meuth, Pierre Maitrias, Jean-Régis Humbert, Benjamin Brigant, et al.. miR-92a: A Novel Potential Biomarker of Rapid Aortic Valve Calcification. Journal of Heart Valve Disease, 2017, 26 (3), pp.327-333. ⟨hal-04076106⟩