Determination of the Antioxidant and Antiproliferative Properties of Pomegranate Peel Extract Obtained by Ultrasound on HCT-116 Colorectal Cancer Cell Line - Université de Picardie Jules Verne Accéder directement au contenu
Article Dans Une Revue Processes Année : 2023

Determination of the Antioxidant and Antiproliferative Properties of Pomegranate Peel Extract Obtained by Ultrasound on HCT-116 Colorectal Cancer Cell Line

Christie Habchi
  • Fonction : Auteur
Adnan Badran
  • Fonction : Auteur
Mortada Srour
  • Fonction : Auteur
Anis Daou
Elias Baydoun
  • Fonction : Auteur
Kamar Hamade
  • Fonction : Auteur
  • PersonId : 1256931
  • IdRef : 264919254

Résumé

Colorectal cancer (CRC) is one of the most common cancers in the world. When treating patients, therapeutic agents have side effects; hence, the use of natural compounds found in medicinal plants including pomegranate. Ultrasound assisted extraction (UAE) is a new technique evolving to the detriment of traditional methods such as maceration. In this study, we investigated the antioxidant and anticancer effect of pomegranate peel extracts obtained by maceration and UAE at three different ultrasonic power levels (P1 = 10 W; P2 = 50 W; P3 = 100 W) on HCT-116 colorectal cancer cells. Phytochemical screening highlighted the presence of primary and secondary metabolites in pomegranate peels. In addition, the ethanolic extract obtained by UAE at 50 W was shown to be the most concentrated in phenolic and flavonoid compounds and have the most powerful antioxidant activity, which reached a maximum activity of 92% as determined by DPPH test. Similarly, the MTT cell viability test showed that the extract obtained by UAE at 50 W had the most potent inhibitory effect compared to the other extracts. In conclusion, the UAE at 50 W was shown to be the most suitable and efficient extraction technique to obtain bioactive compounds from pomegranate peel extracts that can be used in the treatment of CRC.

Dates et versions

hal-04106552 , version 1 (25-05-2023)

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Citer

Christie Habchi, Adnan Badran, Mortada Srour, Anis Daou, Elias Baydoun, et al.. Determination of the Antioxidant and Antiproliferative Properties of Pomegranate Peel Extract Obtained by Ultrasound on HCT-116 Colorectal Cancer Cell Line. Processes, 2023, 11 (4), pp.1111. ⟨10.3390/pr11041111⟩. ⟨hal-04106552⟩
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