Tropane alkaloids GC/MS analysis and low dose elicitors' effects on hyoscyamine biosynthetic pathway in hairy roots of Algerian Datura species - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Scientific Reports Année : 2018

Tropane alkaloids GC/MS analysis and low dose elicitors' effects on hyoscyamine biosynthetic pathway in hairy roots of Algerian Datura species

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Boualem Harfi
  • Fonction : Auteur
Lakhdar Khelifi
Majda Khelifi-Slaoui
  • Fonction : Auteur

Résumé

Plant secondary metabolites play a major role in plant adaptation to stress. Species belonging to Solanaceae family and Datura genus produce tropane alkaloids: D. stramonium, D. tatula and D. innoxia. These species are cultivated for their hyoscyamine (HS) content, whence the interest of this study to induce transformed roots of these species with strain A4 of Agrobacterium rhizogenes. Hairy roots (HRs) of Datura were established at high frequency by infecting vitroplants. All HRs (343 independent lines) were next employed to study the production of HS and growth. A screening of HRs alkaloid content by GC/MS is performed; it reveals, for the first time, the production of 13 alkaloids by the selected root lines. The selection of high productive line offers an interesting option to enhance the productivity. As HS is the dominant compound, the lines of Datura species were selected for their characteristics for biomass and HS production. The elicitors salicylic acid (SA) and acetyl salicylic acid (ASA) were also used to increase HS production. The results showed that the optimal concentration of the two elicitors (AS and ASA) was 0.1 mM. The highest HS content (17.94 +/- 0.14 mg g(-1) D. W.) obtained in HRs of D. tatula treated with 0.1 mM of acetyl salicylic acid.

Dates et versions

hal-03609454 , version 1 (15-03-2022)

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Citer

Boualem Harfi, Lakhdar Khelifi, Majda Khelifi-Slaoui, Corinne Assaf-Ducrocq, Eric Gontier. Tropane alkaloids GC/MS analysis and low dose elicitors' effects on hyoscyamine biosynthetic pathway in hairy roots of Algerian Datura species. Scientific Reports, 2018, 8, ⟨10.1038/s41598-018-36625-4⟩. ⟨hal-03609454⟩
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