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Article Dans Une Revue Carbohydrate Polymers Année : 2022

Development of phenol-grafted polyglucuronic acid and its application to extrusion-based bioprinting inks

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Takashi Kotani
  • Fonction : Auteur
Ryohei Harada
  • Fonction : Auteur
Ryota Goto
  • Fonction : Auteur
Takahiro Morita
  • Fonction : Auteur
Pascal Dubessay
  • Fonction : Auteur
Guillaume Pierre
  • Fonction : Auteur
Philippe Michaud
Masaki Nakahata
  • Fonction : Auteur
Masaru Kojima
  • Fonction : Auteur
Emmanuel Petit
  • Fonction : Auteur
  • PersonId : 1151920
  • IdRef : 157819418
Cedric Delattre
  • Fonction : Auteur

Résumé

In this present work, we developed a phenol grafted polyglucuronic acid (PGU) and investigated the usefulness in tissue engineering field by using this derivative as a bioink component allowing gelation in extrusion-based 3D bioprinting. The PGU derivative was obtained by conjugating with tyramine, and the aqueous solution of the derivative was curable through a horseradish peroxidase (HRP)-catalyzed reaction. From 2.0 w/v% solution of the derivative containing 5 U/mL HRP, hydrogel constructs were successfully obtained with a good shape fidelity to blueprints. Mouse fibroblasts and human hepatoma cells enclosed in the printed constructs showed about 95% viability the day after printing and survived for 11 days of study without a remarkable decrease in viability. These results demonstrate the great potential of the PGU derivative in tissue engineering field especially as an ink component of extrusion-based 3D bioprinting.

Dates et versions

hal-03609608 , version 1 (15-03-2022)

Identifiants

Citer

Shinji Sakai, Takashi Kotani, Ryohei Harada, Ryota Goto, Takahiro Morita, et al.. Development of phenol-grafted polyglucuronic acid and its application to extrusion-based bioprinting inks. Carbohydrate Polymers, 2022, 277, ⟨10.1016/j.carbpol.2021.118820⟩. ⟨hal-03609608⟩
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