Use of qNMR for speciation of flaxseeds (Linum usitatissimum) and quantification of cyanogenic glycosides

Romain Roulard (1) , Jean-Xavier Fontaine (2) , Arash Jamali (3) , Dominique Cailleu (4) , Reynald Tavernier (5) , Larbi Rhazi (6) , Emmanuel Petit (2) , Roland Molinie (2) , Francois Mesnard (2) , Xavier Guillot (7)

1 UPJV - Université de Picardie Jules Verne
2 BIOPI - Biologie des Plantes et Innovation - UR UPJV 3900
3 LRCS - Laboratoire réactivité et chimie des solides - UMR CNRS 7314
4 LG2A - Laboratoire de Glycochimie, des Antimicrobiens et des Agro-ressources - UMR CNRS 7378
5 Linea
6 Institut Polytechnique LaSalle Beauvais
7 Laboulet Semences S.A.

This report describes a routine method taking less than 20 min to quantify cyanogenic glycosides such as linustatin and neolinustatin from flaxseeds (Linum usitatissimum L.) using H-1 nuclear magnetic resonance. After manual dehulling, a higher linustatin content was shown in the almond fraction, while neolinustatin and total cyanogenic glycoside contents were significantly higher in hulls. Linustatin and neolinustatin were quantified in seven cultivars grown in two locations in three different years. Linustatin, neolinustatin, and total cyanogenic glycosides ranged between 91 and 267 mg/100 g, 78-272 mg/100 g, and 198-513 mg/100 g dry weight flaxseeds, respectively. NMR revealed differences of up to 70% between samples with standard deviation variations lower than 6%. This study shows that NMR is a very suitable tool to perform flaxseed varietal selection for the cyanogenic glycoside content.

Domaines: Sciences du Vivant [q-bio]

Romain Roulard, Jean-Xavier Fontaine, Arash Jamali, Dominique Cailleu, Reynald Tavernier, et al.. Use of qNMR for speciation of flaxseeds (Linum usitatissimum) and quantification of cyanogenic glycosides. Analytical and Bioanalytical Chemistry, 2017, 409 (30), pp.7011-7026. ⟨10.1007/s00216-017-0637-7⟩. ⟨hal-03609609⟩