The Cell Wall Arabinose-Deficient Arabidopsis thaliana Mutant murus5 Encodes a Defective Allele of REVERSIBLY GLYCOSYLATED POLYPEPTIDE2 - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Plant Physiology Année : 2016

The Cell Wall Arabinose-Deficient Arabidopsis thaliana Mutant murus5 Encodes a Defective Allele of REVERSIBLY GLYCOSYLATED POLYPEPTIDE2

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Christopher K. Dugard
  • Fonction : Auteur
Rachel A. Mertz
  • Fonction : Auteur
Davide Mercadante
  • Fonction : Auteur
Christopher Hart
  • Fonction : Auteur
Matheus R. Benatti
  • Fonction : Auteur
Anna T. Olek
  • Fonction : Auteur
Phillip J. Sanmiguel
  • Fonction : Auteur
Bruce R. Cooper
  • Fonction : Auteur
Wolf-Dieter Reiter
  • Fonction : Auteur
Maureen C. Mccann
  • Fonction : Auteur

Résumé

Traditional marker-based mapping and next-generation sequencing was used to determine that the Arabidopsis (Arabidopsis thaliana) low cell wall arabinose mutant murus5 (mur5) encodes a defective allele of REVERSIBLY GLYCOSYLATED POLYPEPTIDE2 (RGP2). Marker analysis of 13 F2 confirmed mutant progeny from a recombinant mapping population gave a rough map position on the upper arm of chromosome 5, and deep sequencing of DNA from these 13 lines gave five candidate genes with G -> A (C -> T) transitions predicted to result in amino acid changes. Of these five, only insertional mutant alleles of RGP2, a gene that encodes a UDP-arabinose mutase that interconverts UDP-arabinopyranose and UDP-arabinofuranose, exhibited the low cell wall arabinose phenotype. The identities of mur5 and two SALK insertional alleles were confirmed by allelism tests and overexpression of wild-type RGP2 complementary DNA placed under the control of the 35S promoter in the three alleles. The mur5 mutation results in the conversion of cysteine-257 to tyrosine-257 within a conserved hydrophobic cluster predicted to be distal to the active site and essential for protein stability and possible heterodimerization with other isoforms of RGP.

Dates et versions

hal-03610851 , version 1 (16-03-2022)

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Citer

Christopher K. Dugard, Rachel A. Mertz, Catherine Rayon, Davide Mercadante, Christopher Hart, et al.. The Cell Wall Arabinose-Deficient Arabidopsis thaliana Mutant murus5 Encodes a Defective Allele of REVERSIBLY GLYCOSYLATED POLYPEPTIDE2. Plant Physiology, 2016, 171 (3), pp.1905-1920. ⟨10.1104/pp.15.01922⟩. ⟨hal-03610851⟩
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