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GEMHEP multicenter quality control study of PCR detection of GB virus C/hepatitis G virus RNA in serum

Abstract : PCR is, to date, the only available tool for the detection of GB virus C (GBV-C) and hepatitis G virus (HGV) RNAs. Twenty-two French laboratories participated in a quality control study to assess the sensitivity and specificity of their procedures. The panel included 13 positive controls and 7 negative controls. The laboratories used either in-house PCR techniques adapted from the literature or partly standardized commercial tests. Three laboratories performed faultlessly with the entire panel. Most laboratories had excellent specificity (100% in 20 of 22 laboratories). Sensitivity was acceptable (85 to 100%) in 15 centers and insufficient (38 to 77%) in 7. As with nonstandardized in-house PCR, the commercial assays gave discrepant performances in different laboratories. These results suggest that laboratories willing to use PCR for detection of GBV-C/HGV RNA for research or diagnostic purposes should participate in multicenter quality control trials.
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https://hal-u-picardie.archives-ouvertes.fr/hal-03613708
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Soumis le : vendredi 18 mars 2022 - 16:30:49
Dernière modification le : vendredi 5 août 2022 - 11:25:51

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M Bogard, C Buffet-Janvresse, J Cantaloube, P Biagini, G Duverlie, et al.. GEMHEP multicenter quality control study of PCR detection of GB virus C/hepatitis G virus RNA in serum. Journal of Clinical Microbiology, American Society for Microbiology, 1997, 35 (12), pp.3298-3300. ⟨10.1128/jcm.35.12.3298-3300.1997⟩. ⟨hal-03613708⟩

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