GEMHEP multicenter quality control study of PCR detection of GB virus C/hepatitis G virus RNA in serum

M Bogard; C Buffet-Janvresse; J Cantaloube; P Biagini; G Duverlie; Sandrine Castelain; J Izopet; M Dubois; C Defer; I Lepot; J Coste; P Marcellin; M Martinot-Peignoux; P Halfon; V Gerolami; L Frangeul; J Pawlotsky; F Roudot-Thoraval; E Dussaix; P Loiseau; N Ravera; P Lewin; J Lamoril; J Lerable; P Lebon

doi:10.1128/jcm.35.12.3298-3300.1997

Article dans une revue

GEMHEP multicenter quality control study of PCR detection of GB virus C/hepatitis G virus RNA in serum

M Bogard C Buffet-Janvresse J Cantaloube P Biagini G Duverlie Sandrine Castelain ¹ J Izopet M Dubois C Defer I Lepot J Coste P Marcellin M Martinot-Peignoux P Halfon V Gerolami L Frangeul J Pawlotsky F Roudot-Thoraval E Dussaix P Loiseau N Ravera P Lewin J Lamoril J Lerable P Lebon

1 AGIR - Agents infectieux, résistance et chimiothérapie - UR UPJV 4294

Abstract : PCR is, to date, the only available tool for the detection of GB virus C (GBV-C) and hepatitis G virus (HGV) RNAs. Twenty-two French laboratories participated in a quality control study to assess the sensitivity and specificity of their procedures. The panel included 13 positive controls and 7 negative controls. The laboratories used either in-house PCR techniques adapted from the literature or partly standardized commercial tests. Three laboratories performed faultlessly with the entire panel. Most laboratories had excellent specificity (100% in 20 of 22 laboratories). Sensitivity was acceptable (85 to 100%) in 15 centers and insufficient (38 to 77%) in 7. As with nonstandardized in-house PCR, the commercial assays gave discrepant performances in different laboratories. These results suggest that laboratories willing to use PCR for detection of GBV-C/HGV RNA for research or diagnostic purposes should participate in multicenter quality control trials.

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Article dans une revue

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Sciences du Vivant [q-bio]

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https://hal-u-picardie.archives-ouvertes.fr/hal-03613708
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GEMHEP multicenter quality control study of PCR detection of GB virus C/hepatitis G virus RNA in serum

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