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Identification of gene expression profiles associated with an Increased risk of post-operative recurrence in Crohn's disease

Abstract : BACKGROUND AND AIMS: Ileocolonic resection is frequently needed in the course of Crohn's disease [CD] treatment and post-operative recurrence is extremely common. Our main objective was to analyse gene expression in the mucosa of CD patients at the time of surgery and at post-operative endoscopy, in order to identify predictors and mechanisms of early endoscopic recurrence. METHODS: We conducted transcriptome analyses on ileal mucosa samples collected from inflamed sections of the surgical specimens [n = 200], from ileal resection margins [n = 149] and in the neo-terminal ileum 6 months after surgery [n = 122]; these were compared with non-inflammatory bowel disease controls [n = 25]. The primary endpoint was post-operative endoscopic recurrence at 6 months. We applied regression models to identify gene signatures predicting endoscopic recurrence. RESULTS: Chronic inflammation was associated with strong expression of inflammatory genes [IL-6, IL-8, IL-1B] and decreased expression of genes involved in metabolic processes, but with a high inter-individual heterogeneity. Gene signatures associated with early endoscopic recurrence were mainly characterized by upregulation of TNFα, IFNγ, IL23A and IL17A. Pathway analyses showed that upregulation of mitochondrial dysfunction within the inflamed sections and JAK/STAT at the ileal margin were predictive of post-operative recurrence. A combined model integrating these top pathway signatures improved the prediction of endoscopic recurrence [area under the curve of 0.79]. STAT3 phosphorylation at the surgical ileal margin was associated with severe recurrence at 6 months. CONCLUSION: We identified several biological pathways in surgical ileal mucosa specimens associated with an increased risk of disease recurrence. Integration of the JAK/STAT and mitochondrial dysfunction pathways in the clinical model improved the prediction of post-operative recurrence.
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https://hal-u-picardie.archives-ouvertes.fr/hal-03773378
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Soumis le : vendredi 9 septembre 2022 - 10:44:19
Dernière modification le : jeudi 15 septembre 2022 - 04:06:06

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Marjolaine Ngollo, Kevin Perez, Nassim Hammoudi, Yuri Gorelik, Marc Delord, et al.. Identification of gene expression profiles associated with an Increased risk of post-operative recurrence in Crohn's disease. Journal of Crohn's & Colitis, Oxford University Press 2022, 16 (8), pp.1269--1280. ⟨10.1093/ecco-jcc/jjac021⟩. ⟨hal-03773378⟩

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