An inter-laboratory multi-centre evaluation of the performance of Mucorales PCR assays when testing serum specimens: A study by the ISHAM Fungal PCR Initiative and the ModiMucor study group -FPCRI Mucor Laboratory Working group

S. Rocchi (1) , Carlo Mengoli (2) , P. White , R. Barnes (3) , J.P. Donnelly , J. Loeffler (4) , F Morio , B Sendid (5) , L Millon (6) , F Dalle (7, 8) , M Lackner , M Cogliati (9) , S Fuchs , A Alanio , W Posch , F Hagen , J Springer , M E Bougnoux , C Klaassen , C Halliday , M Lengerova , S Bretagne (10, 11, 12, 13) , R Hare , Céline Damiani (14) , J Denis , E Scherer , P Poirier , M Gitts-Muselli , X Iriart (15)

1 LCE - Laboratoire Chrono-environnement (UMR 6249)
2 Unipd - Università degli Studi di Padova = University of Padua
3 APL - Johns Hopkins University Applied Physics Laboratory [Laurel, MD]
4 URA 1446 CNRS - Laboratoire de Physiologie Générale [ULP, Strasbourg]
5 INSERM - Institut National de la Santé et de la Recherche Médicale
6 service de parasito-mycologie CHU Besancon
7 Laboratoire de parasitologie mycologie (CHU de Dijon)
8 PAM - Procédés Alimentaires et Microbiologiques
9 UNIMI - Università degli Studi di Milano = University of Milan
10 CNRMA - Centre National de Référence des Mycoses invasives et antifongiques - Mycologie moléculaire
11 Laboratoire de Parasitologie-Mycologie [CHU Saint Louis, Paris]
12 UPD7 - Université Paris Diderot - Paris 7
13 Mycologie moléculaire
14 AGIR - Agents infectieux, résistance et chimiothérapie - UR UPJV 4294
15 CHU Toulouse - Centre Hospitalier Universitaire de Toulouse

In that case the isolate was identified by ITS sequencing. Therefore the present case is the first describing the preliminary identification of Mortierella spp. with a conventional method. It is important to note that the conventional method could identify Mortierella spp. seven days earlier than ITS sequencing. It is still not clear if the present case with Mortierella spp. is colonization or an infection. The patient is currently being followed up clinically and further lower respiratory samples will be taken up. In the laboratory it feels comfortable to have access to both conventional and modem diagnostic methods. The present study describes the importance of hands on experience in diagnosis of rare fungal patogens.

Domaines: Sciences du Vivant [q-bio]

S. Rocchi, Carlo Mengoli, P. White, R. Barnes, J.P. Donnelly, et al.. An inter-laboratory multi-centre evaluation of the performance of Mucorales PCR assays when testing serum specimens: A study by the ISHAM Fungal PCR Initiative and the ModiMucor study group -FPCRI Mucor Laboratory Working group. 9th Trends in Medical Mycology, Oct 2019, Nice, France. pp.P164. ⟨hal-04164225⟩